What is LPCB and its use?
LPCB is a stain used for making semi-permanent microscopic preparation of fungi. The LPCB stain has following three components : Phenol : Kills any live organism. Lactic acid : Preserves fungal structures.
What is LPCB in microbiology?
Lactophenol Cotton Blue (LPCB) Staining is a simple histological staining method used for the microscopic examination and identification of fungi.
What is tease Mount preparation?
Tease mount preparation Put a drop of LPCB on a clean grease-free glass slide. With the help of a needle, tease the fungal culture first and spread it in the LPCB. Wait for normally 30 minutes . i.e. sufficient time for the structures to take up the stain.
What is Riddell slide technique?
The best method for observing sporulation is the slide culture technique described by Riddell in 1950. 1 Riddell’s method required using an agar block of media transferred to a glass slide and placed in a moist chamber. A modified technique developed by Harris in 1986 utilized a layer of water agar to provide humidity.
Why isolation of fungi from soil is required?
Soil fungi were isolated by using dilution plating technique and was identified by Biolog’s Microbial Systems. Both identified fungi were found to be beneficial in enhancing plant growth and increasing the availability of nutrient content in the soil and thus recovering the nutrient content in the flooded soil.
Why is lactophenol used in molds?
Lactophenol cotton blue is a stain that is used to examine fungal elements following either a tape preparation or a scraping. This stain contains phenol, which will kill the organisms, lactic acid which preserves fungal structures, and cotton blue which stains the chitin found in the fungal cell walls.
Why is lactophenol used as a mounting medium?
Remel Lactophenol is a mounting fluid recommended for use in qualitative procedures to prepare fungal specimens for microscopic examination. Phenol precipitates cytoplasmic proteins and inactivates enzyme systems within the fungal cell. Glycerol prevents drying and lactic acid is a clearing agent.
Is PIR LPCB approved?
The Loss Prevention Certification Board (LPCB) and FM Global approved PIR cored panels are generally acceptable to insurers.
What is SR2 rating?
SR2 testing simulates a more determined opportunistic attack. To reach this standard, a piece of security equipment must withstand a 3-minute attack from an assailant equipped with items from tool category B in the LPS 1175 testing manual — this includes claw hammers, hand drills, and junior hacksaws.
What is LPCB wet mount preparation?
The lactophenol cotton blue (LPCB) wet mount preparation is the most widely used method of staining and observing fungi and is simple to prepare. The preparation has three components: Phenol: kills any live organisms; Lactic acid : It preserves fungal structures, and.
What does LPCB stand for?
The lactophenol cotton blue (LPCB) wet mount preparation is the most widely used method of staining and observing fungi and is simple to prepare. The preparation has the following constituents; Fig: Aspergillus spp in LPCB Mount
How do I apply LPCB to my mountant?
Add one or at most two drops of the LPCB before the alcohol dries out Holding the coverslip between the index finger and thumb, touch one edge of the drop of mountant with a coverslip edge and lower gently avoiding air bubbles Make the initial examination using low power objective.
What is the principle of LPCB stain?
Principle. LPCB is a stain used for making semi-permanent microscopic preparation of fungi. The LPCB stain has following three components : Phenol : Kills any live organism. Lactic acid : Preserves fungal structures. Cotton blue : Stains the chitin and cellulose of the fungal cell wall intensely blue.