What stain is used for liver?
Masson’s trichrome stain is among the most common special stains applied to liver specimens. The stain imparts a blue color to collagen against a red background of hepatocytes and other structures.
What is the pathophysiology of Wilson’s disease?
Pathophysiology of Wilson Disease The genetic defect in Wilson disease impairs copper transport. The impaired transport decreases copper secretion into the bile, thus causing the copper overload and resultant accumulation in the liver, which begins at birth.
What does Rhodanine stain?
Rhodanine stain is used in histology to identify copper deposits. The reaction utilizes the property of copper to bind with high affinity to protein copper deposits in tissue sections.
Does mushroom cause liver disease?
Amanita phalloides is the most common and fatal cause of mushroom poisoning. This mushroom contains amanitins, which are powerful hepatotoxins that inhibit RNA polymerase II in liver. Mushroom poisoning is a relatively rare cause of acute liver failure.
What is reticulin in liver?
Reticulin is used to assess liver architecture. It highlights the reticulin fibers (type III collagen) in the space of Disse, which helps to show the thickness of hepatocyte plates. Further, reticulin makes it easier to visualize areas of hepatocyte loss (collapse) or regeneration (increased thickness).
How does Wilson’s disease affect the liver?
The liver of a person who has Wilson Disease does not release copper into bile as it should. As the copper builds up in the liver, it begins to damage the organ. After enough damage, the liver releases the copper directly into the bloodstream, which carries the copper throughout the body.
What is Orcein stain?
Orcein stain is a staining technique used for the visualization of hepatitis B surface antigens (HBsAg), elastic fibers and copper-bound proteins. With orcein staining, HBsAg antigens appear as irregular aggregates in the cytoplasm of host cells.
What does a rhodanine stain look for?
Rhodanine is a special stain used to evaluate copper (copper associated protein) within hepatocytes. Normal hepatocytes show no increased copper deposition. In conditions with increased copper deposition, such as chronic cholestatic liver disease or Wilson’s disease, the copper appears as orange-brown granules by rhodanine stain (Figure 14).
What is the significance of rhodanine stain in nodular regenerative hyperplasia?
Reticulin stain in nodular regenerative hyperplasia (NRH) showing the nodularity is due to reticulin collapse, not fibrosis. Rhodanine is a special stain used to evaluate copper (copper associated protein) within hepatocytes.
What stain is used for copper in liver biopsy?
Since copper in high concentrations is the specific causal factor in WD, several special stains (Table 23.1) for copper and copper-binding protein increase the diagnostic accuracy of the liver biopsy: the rhodanine method gives the most reliable results [22]. Staining for copper and copper-binding protein may occasionally be dissociated.
What is the DMAB-rhodanine staining protocol?
Rhodanine Staining Protocol. DMAB-rhodanine is a bidentate chelating agent, which has a strong affinity for proteinaceous copper deposits in tissue sections. Increased amounts of copper are found in the liver in several disease states including Wilson’s disease (an inherited condition), the excess copper has a toxic effect causing liver damage…