Where is PDK1 found?
human chromosome 16p
The 556-residue monomeric enzyme comprises a catalytic domain that is most similar to the PKA, PKB and PKC subfamily of protein kinases and a carboxy-terminal pleckstrin homology (PH) domain. The PDK1 gene is located on human chromosome 16p 13.3 and is expressed ubiquitously in human tissues.
What is a kinase consensus sequence?
Protein kinases typically bind and phosphorylate serine, threonine, or tyrosine residues within a distinct consensus sequence. Of these kinases, Akt is a perfect example of how PTMs within a consensus sequence can alter substrate binding.
What is a consensus phosphorylation site?
The amino acids surrounding the site make up the consensus site. In eukaryotes, phosphorylation on serine and threonine residues often occurs when the residue(s) lies near one or more basic amino acids. In contrast, tyrosine phosphorylation occurs when the residue is in close proximity to acidic amino acids.
Is AKT a gene?
AKT1 (AKT Serine/Threonine Kinase 1) is a Protein Coding gene. Diseases associated with AKT1 include Proteus Syndrome and Cowden Syndrome 6. Among its related pathways are Regulation of TP53 Activity and DNA Damage Response (only ATM dependent).
Are PKB and Akt the same?
The Akt signaling pathway or PI3K-Akt signaling pathway is a signal transduction pathway that promotes survival and growth in response to extracellular signals. Key proteins involved are PI3K (phosphatidylinositol 3-kinase) and Akt (protein kinase B).
Is Akt a protein kinase B?
Protein kinase B or Akt (PKB/Akt) is a serine/threonine kinase, which in mammals comprises three highly homologous members known as PKBalpha (Akt1), PKBbeta (Akt2), and PKBgamma (Akt3). PKB/Akt is activated in cells exposed to diverse stimuli such as hormones, growth factors, and extracellular matrix components.
How do you find the protein consensus sequence?
A consensus sequence is determined by aligning many nucleotide (or protein) sequences that share a common function, then determining the most commonly expressed nucleotide (or amino acid) at each position. Often conserved sequences reflect a common function or binding domain.
What is the PDK1 gene?
PDK1 (Pyruvate Dehydrogenase Kinase 1) is a Protein Coding gene. Diseases associated with PDK1 include Chronic Mountain Sickness . Among its related pathways are Development EPO-induced Jak-STAT pathway and Factors and pathways affecting insulin-like growth factor (IGF1)-Akt signaling .
What is the difference between PDK1 and polyclonal antibodies?
PDK1 Antibody detects endogenous levels of total PDK1 protein. Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding the carboxy terminus of human PDK1. Antibodies are purified by protein A and peptide affinity chromatography.
What are the GO annotations related to the PDK2 gene?
Gene Ontology (GO) annotations related to this gene include protein kinase activity and pyruvate dehydrogenase (acetyl-transferring) kinase activity . An important paralog of this gene is PDK2.
Where is PDK1 overexpressed in humans?
PDK1 This gene is overexpressed in Stomach (14.0), Peripheral blood mononuclear cells (13.4), and Fetal heart (12.5). Tissue specificity: Expressed predominantly in the heart.